WebOct 7, 2002 · For determining survival of mouse cells, cisplatin was added 24 h after plating of cells (80% confluence) and incubated for 2 h. Cells were detached with 10 mM EDTA in PBS and stained with trypan blue to detect dead cells; unstained cells were counted using a hemocytometer. WebAbstract Cisplatin (DDP) is a common therapeutic option for non-small cell lung …
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Web200±20g Sprague Dawley rats were injected with 6mg/kg cisplatin at 0 day, with none treated rats used as control.24h after injection, equal volume PBS and 1×106MSCs dissolved in PBS were administrated by vein casual seperately.Tissues were colleced at 5 day. Extracted molecule total RNA Extraction protocol WebCisplatin is an antineoplastic agent that produces a number of dose-limiting side effects, … dangers of lidocaine injections
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WebApr 10, 2024 · Acetic acid glacial (99.7%), cisplatin (cis-dichlorodiammine platinum (II), 99.9%), (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Dulbecco’s Modified Eagle’s Medium—high-glucose cell growth medium (DMEM-HG), 1:1 mixture of Dulbecco’s Modified Eagle’s Medium and Ham’s F12 cell growth medium (DMEM/F12 … WebCisplatin is a chemotherapy drug which is used to treat cancers including: sarcoma, … WebDec 8, 2024 · Expression levels of p53 and p27 proteins were determined in CT26 tumors of the three cisplatin-treated BALB/c substrains, using specific antibodies and an imaging densitometer. The levels of each protein are presented relative to the intensity of actin. birmingham to portsmouth coach